Table 1 Results from CGH, breakpoint sequencing, TAIL-PCR, and resequencing of transgenic plants
Transgenic Genotype | Construct | Data Types | Background | CGH-detected SV | T-DNA Locationa | T-DNA Direction | SV adjacent to T-DNA | No. Transgenes |
|---|---|---|---|---|---|---|---|---|
WPT_384-1-1 | TALEN | CGH, TAIL-PCR | ‘Bert-MN-01’ | 23,406 bp; Gm01 deletion | Gm07:35,729,562 | + | Untested | Likely 1 |
WPT_389-2-2 | mPing-Pong Transposon | CGH, NGS, TAIL-PCR, Southern Blot | ‘Bert-MN-01’ | 125,228 bp; Gm11 deletion | Gm13:35,614,273 | + | 1,533 bp deletion + 37 bp deletion | 1 |
WPT_301-3-13 | GFP + RNAi Hairpin | CGH, TAIL-PCR, Southern Blot | ‘Wm82-ISU-01’ | 6,869 bp; Gm13 duplication | Gm04:2,695,263 | - | Untested | 1 |
WPT_391-1-6 | Magnesium Chelatase RNAi Hairpin | CGH, NGS | ‘Bert-MN-01’ | 7,854 bp; Gm19 deletion | Gm05:38,834,281 | + | ~1,200 bp deletion | 1 |
WPT_312-5-126 | Zinc Finger Nuclease | CGH, Southern Blot | ‘Bert-MN-01’ | None | Untested | NA | Untested | 1 |