Fig. 5

Transgene insertion locus and induced homozygous deletions in transgenic plant WPT_389-2-2. a A graphical interpretation of the transgene orientation and induced deletions at this locus is shown. The transgene insertion on chromosome 13 contains four primary elements between the left and right borders: Pong, mPing, Tpase, and BAR. Colored lines correspond to the breakpoint sequence results. b Results of breakpoint sequencing show a 1,533 bp deletion adjacent to the T-DNA right border (dark blue). The deletion results in a unique junction connecting two genomic segments (red and green) immediately adjacent to a 6 bp track of filler sequence (light blue), and then the T-DNA right border (dark blue). c A 37 bp deletion is found at the left border-genome junction (orange and purple, respectively). Microhomology occurs across the large deletion and between the left border and the genome