Table 1 Strains and plasmids used in the current study
| Â | Description | Source |
|---|---|---|
Strains | Â | Â |
  Burkholderis sp. ZYB002 | Wild-type, lipase-producing strain with multiple antibiotic resistance | Shu et al., 2009 [14] |
  Burkholderis sp. ZYB002 -ΔlipA | lipA-inactivation mutant strain derived from Burkholderis sp. ZYB002; Tmpr and lipA::gfp | This study |
  Burkholderis sp. ZYB002 -ΔlipC24 | lipC24-inactivation mutant strain derived from Burkholderis sp. ZYB002; Tmpr and lipC24::gfp | This study |
  E. coli DH5α | fhuA2 lac(del)U169 phoA glnV44 Φ80’ lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | TAKARA |
  E. coli BL21(DE3) | Expression host strain for lipA and lipC21 | Novagen |
  E. coli Origami2 (DE3) | Expression host strain for lipC24 | Novagen |
Plasmids | Â | Â |
 pMD18T-lipAB | pMD18T containing the PCR-amplified lipA and lipB | This study |
 pMD18T-lipC21 | pMD18T containing the PCR-amplified lipC21 | This study |
 pMD18T-lipC24 | pMD18T containing the PCR-amplified lipC24 | This study |
 pEDSF-lipB | pACYCDuet-1 with insertion of lipB at MCS2 | This study |
 pEDSF-lipB-lipA | pEDSF-lipB with insertion of lipA at MCS1 | This study |
 pEDSF-lipC21 | pET28a with insertion of lipC21 at MCS | This study |
 pEDSF-lipB-lipC24 | pEDSI-lipB with insertion of lipC24 at MCS1 | This study |
 pGro7 | Expression plasmid containing groES-groEL gene, araB promoter | TAKARA |
 pBBR1TP | Broad host range cloning vector with the trimethoprim resistance gene | Yingrun Bio. Inc. |
 pJQ200SK | Suicide vector with gentamicin resistance gene | Yingrun Bio. Inc. |
 pRK2013 | The helper plasmid with RK2 transfer genes and kanamycin resistance gene | Yingrun Bio. Inc. |
 pEGFP-N1 | Expression vector with gfp gene and kanamycin resistance gene | Clontech. |
 pBCMB-S1 | pJQ200SK containing the PCR-amplified tmp gene from pBBR1TP | This study |
 pBCMB-S2 | pBCMB-S1 containing the PCR-amplified lipA gene fragment (lipA ,) | This study |
 pBCMB-S3 | pBCMB-S2 containing the PCR-amplified gfp gene from pEGFP-N1 | This study |
 pBCMB-S4 | pBCMB-S1 containing the PCR-amplified lipC24 gene fragment (lipC24 ,) | This study |
 pBCMB-S5 | pBCMB-S4 containing the PCR-amplified gfp gene from pEGFP-N1 | This study |