Table 4 PCR primers designed for plasmids construction
From: Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose
Oligonucleotide primers | Sequences |
|---|---|
‘tesA_ F_NdeI | GGAATTCCATATGGCGGACACGTTATTGATTCTGGG |
‘tesA_R_BglII | GAAGATCTTATGAGTCATGATTTACTAAAGGC |
A1_ F_NcoI | CATGCCATGGGCATGACAATTAAAGAAATGCCTCAG |
A1_ R_EcoRI | CCGGAATTCTTACCCAGCCCACACGTCTTTTG |
T7'tesA_F_EcoRI | CCGGAATTCTAATACGACTCACTATAGGGG |
T7′tesA_R_XhoI | CCGCTCGAGTTATGAGTCATGATTTACTAAAGGC |