Fig. 3
From: Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose
Comparison of HFAs production of several different strains under shake-flask conditions. Data were obtained after each strain was induced for 12 h in liquid LB medium supplemented with 20 g/L glucose. BL21/pE-A1’tesA, strain BL21 star(DE3) expressing B. megaterium CYP102A1 and native E. coli ‘tesA; BL21/pE-A1’tesA&pA-acc, further overexpressing native E. coli ACCase; BL21ΔfadD/pE-A1’tesA&pA-acc, knockout of native fadD gene while coexpressing the three enzymes