Fig. 3
From: Production of antigen-specific human IgGs by in vitro immunization
The 22–57 region of Tat suffices to raise an IgG response and preservation of the cysteine residues is absolutely required for the phenomenon. PBMCs were incubated for seven days with or without anti-CD40/IL-4/IL-21, ZZTat101 + anti-CD40/IL-4/IL-21, ZZTat22-57 + anti-CD40/IL-4/IL-21, ZZTat22-57C(22–37)S + anti-CD40/IL-4/IL-21, respectively. Then, supernatants were collected and added to Tat101-coated plates. Presence of anti-Tat IgG was subsequently assessed as described in Fig. 1a