Fig. 2

Sketch of plasmids construction. pET-ABP, a plasmid including NHase encoding genes A (α-subunit), B (β-subunit) and P (P14K); pET-AB(his-P), a plasmid including NHase encoding genes A (α-subunit), B (β-subunit) and his-P (N-terminal his-tagged P14K); pET-AB(P-his), a plasmid including NHase encoding genes A (α-subunit), B (β-subunit) and P-his (C-terminal his-tagged P14K); pET-AB(gst-P), a plasmid including NHase encoding genes A (α-subunit), B (β-subunit) and gst-P (N-terminal gst-tagged P14K); pET-(A*)BP, a plasmid including NHase encoding genes A* (mutant α-subunit with Ser115 to Ala115), B (β-subunit) and P (P14K)