Fig. 4From: The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris Effect of copper concentrations (0.1 mM to 0.5 mM) on laccase activity. BMDH medium (pH 5.0) was used for growth of P. pastoris. Laccase activity in the supernatant was quantified using the DMPPDA assay. The values are arithmetic mean of two biological experiments. Error bars indicate deviation from the meanBack to article page