Fig. 2

Effect of induction temperature and casamino acids supplementation on the localization of expressed EDIII. Expression was tested at six CA concentrations (0, 0.25, 0.5, 1.0, 1.5, 2.0 % w/v) for 6 days at 20 °C (a) and 30 °C (b). EDIII, viz. secreted (green) and intracellular (red) levels were determined using immunoassay and value inside each bar represents EDIII titers in mg/L broth. Similar patterns were observed from culture supernatant (secretory EDIII) and cell mass (intracellular EDIII) from two independent inductions