Purification, biochemical characterization and structural modelling of alkali-stable β-1,4-xylan xylanohydrolase from Aspergillus fumigatus R1 isolated from soil

Table 1 Purification chart of xylanase from Aspergillus fumigatus R1

Purification step	Total activity^a	Total Protein^b	Specific activity	Purification fold	Yield
Purification step	(U)	(mg)	(U/mg)	Purification fold	(%)
Crude extract	176800	253.72	696.8	1	100
30-55 % (NH₄)₂SO₄ Precipitation	43598	6.92	6300.28	9.04	24.6
Bio-Gel P-60 gel- Filtration	6073.2	0.159	38196.22	54.81	3.43

^axylanase activity was measured in 50 mM acetate buffer at 50⁰C for 30 min using 1.0 % (w/v) birchwood xylan as a substrate by the Dinitrosalicylic acid method [13]
^bMeasured by the method of Lowry et al. [34] using bovine serum albumin as a standard

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