Table 2 Primers used in the analysis of PT1-CHO cell lines
Primer designation | Type of analysis | Primer sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
PTl-HC-Fwd | qRT-PCR | GTGAAGGGCCGATTCACTAT | 179 |
PTl-HC-Rev | TTGGCTGAGGAGACTGTGAC | ||
PTl-LC-Fwd | qRT-PCR | GGCACACGGTATTCTCTCAA | 192 |
PTl-LC-Rev | TGAGGCACCTCCAGATGTTA | ||
PTl-LC-Fwd-2 | qPCR | ACCAACCGTATCCATCTTCC | 125 |
PTl-LC-Rev-2 | AACTGTTCAGGACGCCATT | ||
Vezt_305_up | qPCR | TGAACTTGAAAGCTCGTTTG | 134 |
Vezt_305_dw | CTCCGGAGCAGTTTTATCCAC | ||
EEF1A1-B5-Fwd | Bisulfite sequencing | TTGTTGTAGGGAGTTTAAAATGGAG | 231 |
EEFlAl-BS-Rev | TCCACCCACTCAATATAAAAAAACT | ||
EEF1A1-Nuc853-Fwd | ChIP, FAIRE | AGTTGCGTGAGCGGAAAGAT | 111 |
EEF1A1-Nuc853-Rev | CCTTTGTGTGGGTGACT | ||
EEF1A1-Nucl008-Fwd | ChIP, FAIRE | GATTAGTTCTCGAGCTTTTGGAGT | 102 |
EEF1A1-Nucl008-Rev | CCTAACTTCAGTCTCCACCCACT | ||
EEF1A1-Nuc853-1008-Fwd | ChIP, FAIRE | GTGAGTCACCCACACAAAGG | 107 |
EEF1A1-Nuc853-1008-Rev | GCTCGAGAACTAATCGAGGTG | ||
EEF1A1-Nucl008 right-Fwd | ChIP, FAIRE | CACACTGAGTGGGTGGAGAC | 107 |
EEFlAl-NuclOOS right-Rev | TGAGGCTTGAGAATGAACCA |