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Table 1 Summary of the different epigenetic results and correlation to recombinant mRNA expression in the PT1-CHO cell lines

From: Mechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell lines

Cell lines mRNA HC/LC (%) ChIP enrichment (% Input) FAIRE M.SssI map (% nucleosome occupancy) % Methylation
H2A H3.3 H2A.Z H3K9ac H3K9me3 H3K4me3 H3K27me3 Nuc 853 Nuc 1008  
PT1-1 1.00 19.50 22.00 5.26 2.17 3.90 8.04 17.20 46.90 60.00 86.67 6.25
PT1-7 53.50 6.67 79.94 135.97 218.15 66.96 5.41 32.23 2.60 60.67 66.67 0.00
PT1-30 22.36 13.31 16.73 11.13 61.99 97.82 17.90 51.18 6.00 73.33 80.00 2.08
PT1-55 104.00 2.93 66.45 7.29 189.00 577.78 16.40 47.40 207.50 87.50 62.50 0.71
Pearson correlation   −0.96 0.76 0.12 0.83 0.91 0.30 0.54 0.77 0.76 −0.95 −0.75
  1. mRNA expression based on ratio qRT-PCR product of heavy-chain primers to light-chain primers (%); ChIP enrichments for histone variants and modifications relative of H2A; FAIRE enrichments (qPCR values using primers described in Materials and Methods); highest score bold and underlined.