Table 3 Effect of detergents on the activity of WT and 1D-5 esterase
Detergents | Relative activity (%) | |||||
|---|---|---|---|---|---|---|
EstS | 1-D5 | |||||
0.5Â % | 1Â % | 5Â % | 0.5Â % | 1Â % | 5Â % | |
Control | 8.8 ± 0.1 U/ml | 11.0 ± 0.2 U/ml | 10.9 ± 0.3 U/ml | 8.4 ± 0.8U/ml | 8.4 ± 0.8U/ml | 8.4 ± 0.8U/ml |
TritonX-100 | 91.4 ± 1.0 | 96.3 ± 2.5 | 90.8 ± 4.5 | 84.3 ± 5.7 | 72.0 ± 6.0 | 39.1 ± 0.6 |
Tween-20 | 99.3 ± 1.7 | 97.7 ± 1.7 | 96.3 ± 3.8 | 86.5 ± 5.3 | 55.4 ± 3.2 | 45.8 ± 1.3 |
Tween-80 | 96.3 ± 2.2 | 98.4 ± 0.2 | 96.1 ± 5.5 | 90.9 ± 4.4 | 88.8 ± 3.4 | 83.4 ± 3.6 |
CHAPS | 110.1 ± 2.9 | 103.8 ± 5.0 | 3.1 ± 6.4 | 76.2 ± 5.8 | 46.6 ± 5.2 | 26.9 ± 1.3 |
SDS | ND | ND | ND | ND | ND | ND |