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Table 3 Effect of detergents on the activity of WT and 1D-5 esterase

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Detergents Relative activity (%)
EstS 1-D5
0.5 % 1 % 5 % 0.5 % 1 % 5 %
Control 8.8 ± 0.1 U/ml 11.0 ± 0.2 U/ml 10.9 ± 0.3 U/ml 8.4 ± 0.8U/ml 8.4 ± 0.8U/ml 8.4 ± 0.8U/ml
TritonX-100 91.4 ± 1.0 96.3 ± 2.5 90.8 ± 4.5 84.3 ± 5.7 72.0 ± 6.0 39.1 ± 0.6
Tween-20 99.3 ± 1.7 97.7 ± 1.7 96.3 ± 3.8 86.5 ± 5.3 55.4 ± 3.2 45.8 ± 1.3
Tween-80 96.3 ± 2.2 98.4 ± 0.2 96.1 ± 5.5 90.9 ± 4.4 88.8 ± 3.4 83.4 ± 3.6
CHAPS 110.1 ± 2.9 103.8 ± 5.0 3.1 ± 6.4 76.2 ± 5.8 46.6 ± 5.2 26.9 ± 1.3
SDS ND ND ND ND ND ND
  1. Data are given as mean values ± SD. The activity without any additives was defined as 100 %. ND means not detected