Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12896-016-0235-3/MediaObjects/12896_2016_235_Fig3_HTML.gif)
Substrate specificity of the purified EstS. The esterase activity of EstS was tested with various chain lengths of p-NP esters (C2, C4, C6, C8, C12 and C16) in 50 mM Tris – HCl, pH 8.5, at 30 °C. The activity against p-NP acetate (C2) was taken as 100 %. All measurements were performed in triplicate