Fig. 3From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolutionSubstrate specificity of the purified EstS. The esterase activity of EstS was tested with various chain lengths of p-NP esters (C2, C4, C6, C8, C12 and C16) in 50 mM Tris – HCl, pH 8.5, at 30 °C. The activity against p-NP acetate (C2) was taken as 100 %. All measurements were performed in triplicateBack to article page