Table 1 Knock-in into the mouse Rosa26 locus using sgRNA and Cas9 mRNA
From: Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
Donor vector | Concentration (ng/μl) | Injected zygotes | Transferred embryos | Live pups (%) | Deletion alleles (%) | Knock-in alleles (%) |
|---|---|---|---|---|---|---|
Rosa26LSL-Cas9 | 10 | 105 | 60 | 7 (12) | 3 (43) | 0 (0) |
20 | 183 | 147 | 31 (21) | 25 (80) | 5 (16) | |
Rosa26LSL-Lgals-Cd274 | 20 | 142 | 96 | 10 (10) | ND | 2 (20) |