Fig. 5
From: Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
Analysis of off-target activity. a: The top 18 predicted off-target sites of the Rosa26-1 target sequence sorted according to sequence divergence (upper panel) and the PCR scheme for the analysis of the top 3 off-targets (lower panel). Negligible mismatches are shown in grey. b: PCR amplification of the off-target site 1 (Off1) from two F1 pups each derived from the mutant founders #18, #35 or #39 (upper panel) and sequencing results of the respective bands (lower panel, Bl6-C57Bl/6 wildtype control). c: PCR amplification of the off-target site 2 (Off2) from two F1 pups each derived from the mutant founders #18, #35 or #39 (upper panel) and sequencing results of the respective bands (lower panel, Bl6-C57Bl/6 wildtype control). d: PCR amplification of the off-target site 3 (Off3) from two F1 pups each derived from the mutant founders #18, #35 or #39 (upper panel) and sequencing results of the respective bands (lower panel, Bl6-C57Bl/6 wildtype control)