Fig. 7

Production and sequence analysis of the obtained MAbs. a Coomassie staining of the supernatant (10 μl) collected after 10 days culture of CHOEBNALT85 cells transfected with the anti-BDNF IgG plasmid vector. Heavy and light chains and assembled IgG molecule are indicated in reduced (DTT+) and non-reduced (DTT-) sample conditions, respectively. Concentration dependent ELISA of the same antibody batch is represented by the diagram using coating with the BDNF or a non-relevant control protein. b Seven Mabs recognizing the C-terminal DNA binding domain of the HPV18 E2 protein were derived from immunized chicken. VH and VL protein sequence analysis revealed that their variable domains were formed using a limited number of CDR sequences. The same color in a particular CDR position (CDR1, 2 or 3 in the VH or VL, respectively) indicates an identical CDR sequence. The number of identified clones with this particular CDR pattern is shown at left (#). c Variable domain sequences of anti-artemin MAbs developed by the method from the spleen of immunized mice. The same color code is used as for the ELISA data of the same MAbs shown in the Fig. 4e and f. CDRs are surrounded by boxes