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Fig. 6 | BMC Biotechnology

Fig. 6

From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Fig. 6

Selective development of non-cross-reactive antibodies using a pre-adsorption step. a Global alignment of the mature protein sequences of human ribonuclease 8 (R8, UniProt ID: Q8TDE3) and ribonuclease 7 (R7, UniProt ID: Q9H1E1). b ELISA assay result obtained using supernatants of CHOEBNALT85 cells transfected with 1 μg of library pool DNA derived from splenocytes captured without a pre-adsorption step. c The same ELISA assay using the library pool from splenocytes captured using the pre-adsorption step with R7 protein (100 μg/ml). d The distribution of R7-reactive (R7+), R8-reactive and only R8-reactive (R8+/R7-) scFv-Fc clones retrieved from the pre-adsorbed and control libraries, respectively

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BMC Biotechnology

ISSN: 1472-6750

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