Fig. 1

(a) Effects of alginate concentration. Immobilization conditions: MnP enzyme (0.2-0.7 mg/ml alginate gel), CaCl₂ solution (1 % w/v); at 1 % sodium alginate concentration no beads were formed. (b) Effects of CaCl₂ concentration. Immobilization conditions: MnP solution (0.2-0.7 mg/ml alginate gel), alginate solution (4 % w/v). (c) Effects of ratio of enzyme to alginate. Immobilization conditions: alginate (4 % w/v), CaCl₂ (2 % w/v) (d) Effect of different alginate beads size. Immobilization conditions: alginate (4 % w/v), CaCl₂ (2 % w/v), MnP solution (0.5 mg/ml alginate gel) (e) Control Ca-alginate beads (f) Ca-alginate beads with immobilized MnP