Groups | n | 5-AZA-CdR (μM) | Methylation level (%) | EGFP mRNA level | EGFP fluorescence (%) |
|---|
A0 | 5 | 0.00000 | 91.6 ± 3.5 | 1.00 ± 0.00 | 2.20 ± 0.47 |
A1 | 5 | 0.00016 | 87.8 ± 2.1 | 1.65 ± 0.34 | 7.68 ± 0.87 |
A2 | 5 | 0.00080 | 85.5 ± 2.5 | 1.77 ± 0.26 | 8.75 ± 1.06 |
A3 | 5 | 0.00400 | 79.8 ± 3.6 | 1.92 ± 0.35 | 9.81 ± 1.29 |
A4 | 5 | 0.02000 | 74.5 ± 3.2 | 12.30 ± 0.93 | 11.18 ± 1.39 |
- Note. Cell groups A0 ~ A4 are treated with different concentrations of 5-AZA-CdR. Methylation levels are the concrete methylation levels of the CMV promoter within Hep G2 cell groups with BSP analysis. EGFP mRNA level are the concrete relative level of EGFP mRNA within Hep G2 cell groups with real-time PCR analysis. EGFP fluorescence is the concrete intensity of fluorescence of Hep G2 cell groups with cytometry analysis. There are significant differences for ANOVA analysis of the methylation level between all groups barring groups A0 and A1and groups A1and A2. There are significant differences for ANOVA analysis of the EGFP mRNA level between all groups barring groups A1 and A2, groups A2 and A3, and groups A1 and A3. There are significant differences for ANOVA analysis of the EGFP fluorescence between all groups barring groups A1 and A2, groups A2 and A3, and groups A3 and A4
