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Table 1 The effect of 5-AZA-CdR on the EGFP reporter gene in cells with the TDQ experiment

From: A novel quantification method for the total demethylation potential of aquatic sample extracts from Bohai Bay using the EGFP reporter gene

Groups n 5-AZA-CdR (μM) Methylation level (%) EGFP mRNA level EGFP fluorescence (%)
A0 5 0.00000 91.6 ± 3.5 1.00 ± 0.00 2.20 ± 0.47
A1 5 0.00016 87.8 ± 2.1 1.65 ± 0.34 7.68 ± 0.87
A2 5 0.00080 85.5 ± 2.5 1.77 ± 0.26 8.75 ± 1.06
A3 5 0.00400 79.8 ± 3.6 1.92 ± 0.35 9.81 ± 1.29
A4 5 0.02000 74.5 ± 3.2 12.30 ± 0.93 11.18 ± 1.39
  1. Note. Cell groups A0 ~ A4 are treated with different concentrations of 5-AZA-CdR. Methylation levels are the concrete methylation levels of the CMV promoter within Hep G2 cell groups with BSP analysis. EGFP mRNA level are the concrete relative level of EGFP mRNA within Hep G2 cell groups with real-time PCR analysis. EGFP fluorescence is the concrete intensity of fluorescence of Hep G2 cell groups with cytometry analysis. There are significant differences for ANOVA analysis of the methylation level between all groups barring groups A0 and A1and groups A1and A2. There are significant differences for ANOVA analysis of the EGFP mRNA level between all groups barring groups A1 and A2, groups A2 and A3, and groups A1 and A3. There are significant differences for ANOVA analysis of the EGFP fluorescence between all groups barring groups A1 and A2, groups A2 and A3, and groups A3 and A4