Fig. 3

a Analysis of denaturing IMAC elution fractions by SDS–PAGE. Samples were diluted 1:8 in water and 15 μl of each dilution were loaded per well. b An accompanying chromatogram with two peaks highlighted at the bottom. Peak #1 shows no protein on the gel (lanes 5 and 6) as the amount of this protein was insignificant after dilution to remove guanidine