A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Table 5 The catalytic activity comparison with different temperature

Enzyme	Strain and reference	optimum temperature (°C)	Relative activity at a range of temperature (%)
Enzyme	Strain and reference	optimum temperature (°C)	10	20	30	40	50	60	70
Pul_N	Paenibacillus polymyxa Nws-pp2 (this study)	35	40	70	85	55	<20	—	—
Pul-SH3	Exiguobacterium sp. SH3 [8]	30	45	80	100	70	<40	—	—
rPulAs	Fervidobacterium pennavorans Ven5 [16]	80	—	—	<10	20	35	50	80
rPulAg	Anaerobranca gottschalkii [12]	70	—	—	—	<30	55	85	100
pulullanase	Bacillus sp. AN-7 [27]	90	—	—	—	<25	50	65	80
pulullanase	Bacillus stearothermophilus [28]	65	—	<10	25	45	70	95	10
PulA1	Bacillus sp. CICIM 263 [14]	70	—	—	<40	50	60	80	100

ISSN: 1472-6750