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Table 3 Effects of metal ions and chemical reagents on the PulN activity

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Metal ions compounds

Relative activity (%)

1 mM

5 mM

10 mM

None

100.0

100.0

100.0

MgCl2

104.1 ± 1.4

109.0 ± 0.2

117.0 ± 0.5

CaCl2

97.9 ± 2.0

97.4 ± 1.6

96.0 ± 1.6

CuCl2

75.1 ± 2.5

11.9 ± 0.5

2.9 ± 0.1

ZnSO4

97.6 ± 0.7

83.8 ± 0.7

49.3 ± 1.4

NiSO4

99.0 ± 2.3

103.1 ± 0.2

102.1 ± 0.9

MnCl2

114.8 ± 2.0

154.8 ± 2.7

215.2 ± 4.8

CoCl2

121.7 ± 0.7

124.2 ± 1.1

125.1 ± 0.9

FeSO4

77.2 ± 3.4

58.3 ± 0.9

42.0 ± 1.1

  1. The data represent the mean of three experimental repeats with SD of ≤ 5 %