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Table 3 Effects of metal ions and chemical reagents on the PulN activity

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Metal ions compounds Relative activity (%)
1 mM 5 mM 10 mM
None 100.0 100.0 100.0
MgCl2 104.1 ± 1.4 109.0 ± 0.2 117.0 ± 0.5
CaCl2 97.9 ± 2.0 97.4 ± 1.6 96.0 ± 1.6
CuCl2 75.1 ± 2.5 11.9 ± 0.5 2.9 ± 0.1
ZnSO4 97.6 ± 0.7 83.8 ± 0.7 49.3 ± 1.4
NiSO4 99.0 ± 2.3 103.1 ± 0.2 102.1 ± 0.9
MnCl2 114.8 ± 2.0 154.8 ± 2.7 215.2 ± 4.8
CoCl2 121.7 ± 0.7 124.2 ± 1.1 125.1 ± 0.9
FeSO4 77.2 ± 3.4 58.3 ± 0.9 42.0 ± 1.1
  1. The data represent the mean of three experimental repeats with SD of ≤ 5 %