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Fig. 5 | BMC Biotechnology

Fig. 5

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Fig. 5

Substrate specificity and analysis of hydrolyzed products. a Relative activity on different substrates. b TLC analysis of hydrolysis products with various substrates. Lane G1: glucose; lane G2: maltose; lane G3: maltotriose; lane 1: pullulan with PulN; lane 2: pullulan; lane 3: starch with PulN; lane 4: starch; lane 5: amylopectin with PulN; lane 6: amylopectin; lane 7: glycogen with PulN; lane 8: glycogen; lane 9: products of pullulan with α-glucosidase. c TLC analysis of hydrolysis products compared with the industrial enzyme at different temperature. Lane G1: glucose; lane G2: maltose; lane G3: maltotriose; lane 1: pullulan; lane 2: pullulan with PulN at 0 °C; lane 3: pullulan with Promozyme at 0 °C; lane 4: pullulan with PulN at 20 °C; lane 5: pullulan with Promozyme at 20 °C; lane 6: pullulan with PulN at 40 °C; lane 7: pullulan with Promozyme at 40 °C. d HPLC analysis of standard (glucose, maltose, maltotriose). e HPLC analysis of hydrolysates from with various substrates (pullulan, soluble starch and amylopectin)

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BMC Biotechnology

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