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Cloning of CaPAO gene
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EST-CaPAOF
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CTTCACTCCCAATCACCAAACC
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EST-CaPAOR
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TCGCTAACGAGCCCAAGATAAT
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5′RACE-CaPAOGSP
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CGGAGACTGAAACGCTTTAGCTTC
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5′RACE-CaPAONGSP
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GAACAACCCACTGAGACCCAGATTT
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3′RACE-CaPAOGSP
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TTGGTGGCAGGTTTTTCCGAGAT
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3′RACE-CaPAONGSP
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CGGCAGCACAGACAACCAACCAC
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quantitative real-time PCR
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RT-CaPAOF
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CAAGTTTGCCTACACCATTTCAG
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RT-CaPAOR
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GCCCATTTTCATCTAATCTCCCT
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CaUBI3F
|
TGTCCATCTGCTCTCTGTTG
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CaUBI3R
|
CACCCCAAGCACAATAAGAC
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Virus-induced gene silencing (VIGS) vector construction
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TRV-CaPAOF
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CAATGGCACCAGGAAAGACC
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TRV-CaPAOR
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GCGTTTGGACAAGACGGTAG
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CaPDSF
|
TGTTGTCAAAACTCCAAGGTCTGTA
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CaPDSR
|
TTTCTCCCACTTGGTTCACTCTTGT
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Tabacco genetic transformation
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2307-CaPAOF
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ATGGCTTCTTCTTCTTCTTGTAT
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2307-CaPAOR
|
TTCTAAATTTCCCTAGACTTCTAGTC
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RD29A-F
|
GAATAAATATCATACCGACATCA
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CaPAO-R
|
CAGTACTAGTAGATGATTCTTGTTC
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NtUBIF
|
TCCAGAAAGAGTCAACCCG
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NtUBIR
|
GACCTCAGTAGACAAAGCACAT
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