Table 1 Primer sequences in this study
primer | Sequence (5′-3′) |
|---|---|
Cloning of CaPAO gene | |
 EST-CaPAOF | CTTCACTCCCAATCACCAAACC |
 EST-CaPAOR | TCGCTAACGAGCCCAAGATAAT |
 5′RACE-CaPAOGSP | CGGAGACTGAAACGCTTTAGCTTC |
 5′RACE-CaPAONGSP | GAACAACCCACTGAGACCCAGATTT |
 3′RACE-CaPAOGSP | TTGGTGGCAGGTTTTTCCGAGAT |
 3′RACE-CaPAONGSP | CGGCAGCACAGACAACCAACCAC |
quantitative real-time PCR | |
 RT-CaPAOF | CAAGTTTGCCTACACCATTTCAG |
 RT-CaPAOR | GCCCATTTTCATCTAATCTCCCT |
  CaUBI3F | TGTCCATCTGCTCTCTGTTG |
  CaUBI3R | CACCCCAAGCACAATAAGAC |
Virus-induced gene silencing (VIGS) vector construction | |
 TRV-CaPAOF | CAATGGCACCAGGAAAGACC |
 TRV-CaPAOR | GCGTTTGGACAAGACGGTAG |
  CaPDSF | TGTTGTCAAAACTCCAAGGTCTGTA |
  CaPDSR | TTTCTCCCACTTGGTTCACTCTTGT |
Tabacco genetic transformation | |
 2307-CaPAOF | ATGGCTTCTTCTTCTTCTTGTAT |
 2307-CaPAOR | TTCTAAATTTCCCTAGACTTCTAGTC |
 RD29A-F | GAATAAATATCATACCGACATCA |
  CaPAO-R | CAGTACTAGTAGATGATTCTTGTTC |
  NtUBIF | TCCAGAAAGAGTCAACCCG |
  NtUBIR | GACCTCAGTAGACAAAGCACAT |