Table 2 List of primers
Primers | Sequence (5′ → 3′) |
|---|---|
a (or a2) | TCTACTTCTGTTCATGTTTGTG |
a1 | TCTAACCTTGAGTACCTATCTATTA |
b | AAGACCCCAACGAGAAGC |
c | CTCGATGCGATGTTTCGCTT |
d | CTAATCGCCATCTTCCAGCA |
e | ACAGGCTGAACTTGTGGC |
f | GATGGATTGCACGCAGGTTC |
g | GCCACAAGTTCAGCGTGT |
ISceI Foward | GCTGTCTCCTCCTCACAAG |
ISceI Reverse | GGGTCAGGTAGTTCTCCACC |
ZFN Reverse | TGCAGATTCCGACACTGGAAG |
Chr 10 locus1 Forward | GCAAGCCGGTCACCATTTTCA |
Chr 10 locus1 Reverse | TTCGTTGGTTTGCACGCTAT |
Chr 10 locus2 Forward | GAAAGGCGTAACGATCTGGG |
Chr 10 locus2 Forward | TAGTACGAGAGGACCGGGAA |