Table 1 PAM and AP-GLP1-Fc: expression levels and secretion rates. The indicated cell lines were extracted as described in the legend to Fig. 1 and assayed for PHM and PAL activity; data from multiple dilutions of at least 3 independent extracts were averaged and standard errors reported. PHM secretion ( % content/h) was calculated by assaying PHM activity in a 16 h medium collection. Fc levels in cell extracts and spent media were determined by Western blot and used to calculate cell content of Fc and Fc secretion rate

From: Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells