Enhanced UV sensitivity of D. melanica fed on bacteria expressing phenoloxidase dsRNA.
D. melanica were subjected to the RNAi feeding regimen for 10 days and then exposed to UV each day for 2 consecutive days. The percent viability of Daphnia was assessed 24 h after the second exposure. D. pulex was used as a control and all individuals died within 2 h of the initial UV insult. wt melanica (melanica): n = 18, EV: n = 22, GFP (GFP/L4440): n = 24, P1 (P1/L4440): n = 24, P2 (P2/L4440): n = 26, P3 (P3/L4440): n = 30, D. pulex RW20 (pulex): n = 43. These abbreviations denote the plasmid that was transformed into the bacteria before administering them as feed to Daphnia. Data represent 3 replicate experiments. P-values are as follows: * = 0.0293, ** = 0.0028, and *** = 0.0201