Fig. 4
From: Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia
Phenoloxidase transcript levels are diminished in Daphnia fed on bacteria that express phenoloxidase dsRNA. a Reverse Transcriptase PCR examining the expression of phenoloxidase mRNA. RNA samples are as follows- Lane 1: EV (L4440 without any insert), Lane 2: GFP (GFP/L4440), Lane 3: P1 (P1/L4440), Lanes 4: P2 (P2/L4440), Lane 5: P3 (P3/L4440), and Lane 6: non-melanic D. pulex (isolate RW20) control. GAPDH was used as an internal control for ascertaining that equal amounts of Daphnia mRNA were analyzed in each lane. b Quantitative real time PCR examining the expression of phenoloxidase mRNA. The abbreviations under each bar denote which plasmid vector was transformed into the bacteria before administering as feed to Daphnia. P-values are as follows: * = 1 × 10−7, ** = 7.2 × 10-8, and *** = 9.8 × 10−8. Blue bar: EV, Green bar: GFP, red bars: P1, P2, and P3, white bar: non-melanic D. pulex control