Fig. 1
From: Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia
Arthropod Melanin Synthesis pathway and schematic map of regions selected for dsRNA expression constructs in E. coli plasmid L4440. a Arthropod melanin synthesis pathway. b Daphnia phenoloxidase gene and transcript. Marked on each diagram are the regions amplified using PCR and cloned in plasmid L4440. c Schematic diagram of the L4440 plasmid vector. L4440 is designed to produce double stranded RNA of the cloned insert between the two T7 promoters