Fig. 4

Growth and phytase production time course of P. pastoris 6c/HAC1 in a 10-L fermenter. a: Time dependence of phytase activity, cell density and phytase protein content after induction with methanol. The phytase activity was detected using collected culture supernatant. The sample was boiled for 10 min was used as the background sample. b: SDS-PAGE assay of the collected culture supernatant. All the collected supernatants were diluted 1:40 with 100 mM sodium acetate buffer (pH5.5) (v/v). Lane M: protein marker; lane 1: induction for 24 h; lane 2: induction for 48 h; lane 3: induction for 72 h; lane 4: induction for 96 h; lane 5: induction for 120 h; lane 6: induction for 144 h; lane 7: 0.05 mg/mL BSA; lane 8: 0.2 mg/mL BSA; lane 9: 0.3 mg/mL BSA