Fig. 3
From: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris
Different tactics for enhancing the expression of Phy in P. pastoris. a: Effect of modification of P AOX1 and the signal peptide, increasing gene copy number, and overexpression of Kar2p, Ero1p, Pdi1p, or Hac1p on phytase production in recombinant strains carrying six Phy gene copies after 96-h induction with methanol. All activities used GS115/HKA as the background measurement. b: SDS-PAGE assay of the collected culture supernatant from the most effective strains after 96-h induction with methanol. Lane M: protein marker; lane 1: GS115/HKA; lane 2: GS115/Phy; lane 3: GS115/AOXm; lane 4: GS115/αE10; lane 5: GS115/6c; lane 6: 6c/HAC1; lane 7: 0.05 mg/mL BSA; lane 8: 0.2 mg/mL BSA; lane 9: 0.3 mg/mL BSA. c: The phytase protein content of the most effective strains after 96-h induction with methanol