Table 2 Fermentation of four most performing strains expressing MTGase, comparison of two expression systems
From: High-level expression of a recombinant active microbial transglutaminase in Escherichia coli
Fusion protein | Batch # | System | FB/batch | Final OD600 | MTGase (g/batch) | Wet mass (g) |
|---|---|---|---|---|---|---|
LacZ1–8 Met-MTGase | NP676/C002 | Constitutive | Batch | 60 | 26,7 | 693 |
NP676/C001 | Fed-batch | 57 | 24,1 | 709 | ||
NP668/009 | Inducible | Batch | 54 | 25,0 | 540 | |
LacZ1–8PNP1–8 Met-MTGase | NP650/C005 | Constitutive | Batch | 58 | 16,5 | 790 |
NP650/C006 | Fed-batch | 74 | 62,0 | 748 | ||
NP656/C009 | Inducible | Batch | 46 | 39,2 | 335 | |
NP656/C010 | Fed-batch | 56 | 42,7 | 490 |