High-level expression of a recombinant active microbial transglutaminase in Escherichia coli

BMC Biotechnology

Table 1 List of plasmids and related strains, engineered for different MTGase expression forms in Escherichia coli, fermented in shake flask for protein expression detection

Plasmid	Type	Cell strain	Promoter	Cloned gene	Antibiotic resistance
PL444 (Sloning)	Starting	NP647	Lac promoter	Met-MTGase_1–332	Amp
PL447	Expression	NP637	λ-phage	Met-MTGase_1–322	Kan
PL456	Intermediate	NP648	T7-SP6 Promoter	Met-MTGase_1–332	Amp-Kan
PL457	Intermediate	NP649	T7-SP6 Promoter	Met-MTGase_1–332	Amp-Kan
PL458	Expression	NP650	Lac promoter	LacZ_1–8PNP_1–20 Met-MTGase_1–332	Tet
PL463	Intermediate	NP655	Lac promoter	LacZ_1–8Met₋MTGase_1–332	Tet
PL464	Expression	NP656	Phage λ	LacZ_1–8PNP_1–20Met-MTGase_1–332	Kan
PL469	Expression	NP663	Lac promoter	LacZ_1–8	Tet
PL471	Intermediate	NP666	Phage λ	LacZ_1–8Met-MTGase_1–332	Kan
PL472	Expression	NP667	Lac promoter	Met-MTGase_1–332	Tet
PL473	Expression	NP668	Phage λ	LacZ_1–8Met-MTGase_1–332	Kan
PL480	Expression	NP676	Lac promoter	LacZ_1–8Met-MTGase_1–332	Tet

ISSN: 1472-6750