Table 3 Oligonucleotide primers used for amplifying ∆6-desaturase gene of Pythium sp.
Primer name | Oligonucleotide sequence (5′-3′) | Stranda |
|---|---|---|
P29-oligo-dT-AP | GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT | - |
AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | + |
AUAP | GGCCACGCGICGACTAGTAC | + |
P14-PyDes6-F | TTYTGRCARCARTCIGGITGRYTIGCICAYGA | + |
P15-PyDes6-R | GGRAAIARRTGRTGYTCDATYTGRTARTT | - |
P89-PyDes6-R | TTGACGTCGCCGACGAGG | - |
P91-PyDes6-RN | TAGTAGTGGTCGTCCCAGAC | - |
P96-PyDes6-RN | GAGGCCCTTGACCTTGACG | - |
P92-PyDes6-F | AGCAGTTCGCCTTCGGTCG | + |
P93-PyDes6-FN | TGTACGCCAACATGTCCCTG | + |
P94-PyDes6-FN | GCAACATCACGCCGAGTCTC | + |
P99-PyDes6-BamHI-F | CGCGGATCCATGGTGGACCCCAAGCCC | + |
P100-PyDes6-EcoRI-R | CCGGAATTCCTACATGGCCGGGAACTCGG | - |