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Fig. 3 | BMC Biotechnology

Fig. 3

From: An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin

Fig. 3

Protein expression and purification. a Expression of HA1-2. Lanes: M, protein marker; 1, product of BL21(DE3)(pCold) induced by IPTG; 2, product of BL21(DE3)(pCold-HA1-2) not induced; 3, lysate supernatant of BL21(DE3)(pCold-HA1-2) induced by IPTG; 4, inclusion bodies of BL21(DE3)(pCold-HA1-2) induced by IPTG. b Expression of HA1-2-fliC. Lanes: M, protein marker; 1, product of BL21(DE3)(pCold) induced by IPTG; 2, product of BL21(DE3)(pCold-HA1-2-fliC) not induced; 3, lysate supernatant of BL21(DE3)(pCold-HA1-2-fliC) induced by IPTG; 4, inclusion bodies of BL21(DE3)(pCold-HA1-2-fliC) induced by IPTG. c Protein purification. Lanes: M, protein marker; 1, HA1-2; 2, HA1-2-fliC

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BMC Biotechnology

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