Skip to main content

Table 1 Enzymatic properties of activated pro-TGase and its variants

From: Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization

  Wild type N160Q N355Q N160Q/N355Q
t 1/2 (37 °C) (min) 34.0 ± 0.2 24.2 ± 0.1 19.2 ± 0.2 11.5 ± 0.2
Specific activity (U/mg) 16.6 ± 0.2 14.7 ± 0.5 13.3 ± 0.3 10.4 ± 1.3
K m (mmol/L) 59.5 ± 2.3 47.0 ± 1.5 54.7 ± 2.1 48.4 ± 2.4
k cat (s−1) 31.5 ± 1.7 25.3 ± 2.2 23.8 ± 3.2 16.6 ± 1.7
  1. Note: t 1/2 (37 °C) was determined at an enzyme concentration of approximately 0.5 mg/mL for each enzyme; the specific activity measurements were performed using N-CBZ-Gln-Gly as the substrate (30 mmol/L); and kinetic constants were obtained at substrate (N-CBZ-Gln-Gly) concentrations ranging from 6 mmol/L to 30 mmol/L