Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization

Table 1 Enzymatic properties of activated pro-TGase and its variants

	Wild type	N160Q	N355Q	N160Q/N355Q
t _1/2 (37 °C) (min)	34.0 ± 0.2	24.2 ± 0.1	19.2 ± 0.2	11.5 ± 0.2
Specific activity (U/mg)	16.6 ± 0.2	14.7 ± 0.5	13.3 ± 0.3	10.4 ± 1.3
K _m (mmol/L)	59.5 ± 2.3	47.0 ± 1.5	54.7 ± 2.1	48.4 ± 2.4
k _cat (s⁻¹)	31.5 ± 1.7	25.3 ± 2.2	23.8 ± 3.2	16.6 ± 1.7

Note: t _1/2 (37 °C) was determined at an enzyme concentration of approximately 0.5 mg/mL for each enzyme; the specific activity measurements were performed using N-CBZ-Gln-Gly as the substrate (30 mmol/L); and kinetic constants were obtained at substrate (N-CBZ-Gln-Gly) concentrations ranging from 6 mmol/L to 30 mmol/L

ISSN: 1472-6750