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Table 1 Purification of A20L from E. coli Rosetta (DE3) cells expressing Ub-A20L and SUMO1/2/3/4-A20L in a batch culture of 1 L

From: Prokaryotic expression and mechanism of action of α-helical antimicrobial peptide A20L using fusion tags

Protein

Target protein after 1 mM IPTG induction (mg)a

Ni-NTA pool (mg)b

CM Sepharose FF pool (mg)b

A20L (mg)c

His6-Ub-A20L

76.3

51.2

46.5

7.3

His6-SUMO1-A20L

52.0

33.8

30.6

5.4

His6-SUMO2-A20L

48.7

29.7

24.1

3.2

His6-SUMO3-A20L

61.5

36.4

32.9

4.8

His6-SUMO4-A20L

21.9

13.6

10.2

2.0

  1. aThe quantity of target protein was estimated by SDS gel scanning
  2. bThe quantity of target protein was determined by the BCA method
  3. cThe quantity of A20L was determined by the Walker method [15]
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BMC Biotechnology

ISSN: 1472-6750

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