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Table 1 Purification of A20L from E. coli Rosetta (DE3) cells expressing Ub-A20L and SUMO1/2/3/4-A20L in a batch culture of 1 L

From: Prokaryotic expression and mechanism of action of α-helical antimicrobial peptide A20L using fusion tags

Protein Target protein after 1 mM IPTG induction (mg)a Ni-NTA pool (mg)b CM Sepharose FF pool (mg)b A20L (mg)c
His6-Ub-A20L 76.3 51.2 46.5 7.3
His6-SUMO1-A20L 52.0 33.8 30.6 5.4
His6-SUMO2-A20L 48.7 29.7 24.1 3.2
His6-SUMO3-A20L 61.5 36.4 32.9 4.8
His6-SUMO4-A20L 21.9 13.6 10.2 2.0
  1. aThe quantity of target protein was estimated by SDS gel scanning
  2. bThe quantity of target protein was determined by the BCA method
  3. cThe quantity of A20L was determined by the Walker method [15]