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Fig. 2 | BMC Biotechnology

Fig. 2

From: Prokaryotic expression and mechanism of action of α-helical antimicrobial peptide A20L using fusion tags

Fig. 2

(a) Expression of A20L fused with Ub, SUMO1, SUMO2, SUMO3 and SUMO4 proteins. (M): molecular weight marker; (UN): un-induced culture; (IN): induced culture; (Ni): Ni-NTA purified pool; (b) purification of Ub-A20Land SUMO1/2/3/4-A20L by CM Sepharose FF. (M): molecular weight marker; (1) Ub-A20L; (2) SUMO1-A20L; (3) SUMO2-A20L; (4) SUMO3-A20L; (5) SUMO4-A20L; (c) purification of A20L (M): molecular weight marker; (1–5): A20L purified from Ub-A20L(1),SUMO1-A20L(2),SUMO2-A20L(3), SUMO3-A20L(4),SUMO4-A20L(5)

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