Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

BMC Biotechnology

Table 1 Characterization of the yeast-expressed novel HPyV-derived VP1 proteins

Viral origin of VP1	VP1 gene sequence	Length of VP1, aa	Predicted molecular weight, kDa	Formation of VLPs^a	Yield of purified VP1 VLPs from 1 g of wet yeast biomass, mg^b	HA of guinea pig erythrocytes
KIPyV	Optimized	378	41.59	+	0.62 ± 0.05	+
WUPyV	Optimized	369	39.87	+	0.61 ± 0.03	-
MCPyV	Native	423	46.56	+	0,81 ± 0.04	+
HPyV6	Optimized	387	41.84	+	0.55 ± 0.04	-
HPyV7	Optimized	380	40.98	+	0.58 ± 0.03	-
TSPyV	Optimized	375	41.52/	+	1.05 ± 0.04	+
HPyV9	Optimized	371	40,32	+	1.01 ± 0.03	+
HPyV10	Native	403	43.57	+	0.44 ± 0.04	+
STLPyV	Optimized	401	43.54	+	0.62 ± 0.04	-
HPyV12	Optimized	380	42.16	+/−	0.05 ± 0.03	+
HPyV12	Native	364	40.39	+	0.42 ± 0.04	+
HPyV12	Optimized	364	40.39	+	1.02 ± 0.06	+
NJPyV	Optimized	489	53.78	+	0.45 ± 0.03	-

^aThe formation of VLPs was confirmed by negative stain electron microscopy
^bThe values for the yield of VLPs are the average at least from three independent experiments

ISSN: 1472-6750