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Table 1 Characterization of the yeast-expressed novel HPyV-derived VP1 proteins

From: Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

Viral origin of VP1 VP1 gene sequence Length of VP1, aa Predicted molecular weight, kDa Formation of VLPsa Yield of purified VP1 VLPs from 1 g of wet yeast biomass, mgb HA of guinea pig erythrocytes
KIPyV Optimized 378 41.59 + 0.62 ± 0.05 +
WUPyV Optimized 369 39.87 + 0.61 ± 0.03 -
MCPyV Native 423 46.56 + 0,81 ± 0.04 +
HPyV6 Optimized 387 41.84 + 0.55 ± 0.04 -
HPyV7 Optimized 380 40.98 + 0.58 ± 0.03 -
TSPyV Optimized 375 41.52/ + 1.05 ± 0.04 +
HPyV9 Optimized 371 40,32 + 1.01 ± 0.03 +
HPyV10 Native 403 43.57 + 0.44 ± 0.04 +
STLPyV Optimized 401 43.54 + 0.62 ± 0.04 -
HPyV12 Optimized 380 42.16 +/− 0.05 ± 0.03 +
HPyV12 Native 364 40.39 + 0.42 ± 0.04 +
HPyV12 Optimized 364 40.39 + 1.02 ± 0.06 +
NJPyV Optimized 489 53.78 + 0.45 ± 0.03 -
  1. aThe formation of VLPs was confirmed by negative stain electron microscopy
  2. bThe values for the yield of VLPs are the average at least from three independent experiments