Fig. 3
From: Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast
Analysis of HPyV12-derived VP1 VLP production in yeast. a - Analysis of HPyV12 VP1380 VLP production in yeast using Coomassie blue-stained SDS-PAGE. Production of HPyV12 VP1380 protein was analyzed in two yeast clones transformed with pFX7-HPyV12-VP1n380 plasmid (lines 1–4) and two clones transformed with pFX7-HPyV12-VP1s380 plasmid (lines 5–8). In lanes: 1, 3 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n380 plasmid; 2, 4 - the soluble fraction recovered after centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n380 plasmid; 5, 7 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s380 plasmid; 6, 8 - the soluble fraction recovered after the centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s380 plasmid; 9 - purified HPyV12-VP1380 protein; M - pre-stained protein weight marker. b - Analysis of HPyV12 VP1364 production in yeast using Coomassie blue-stained SDS-PAGE. Production of HPyV12 VP1364 protein was analyzed in two yeast clones transformed with pFX7-HPyV12-VP1n364 plasmid (lines 1–4) and two clones transformed with pFX7-HPyV12-VP1s364 plasmid (lines 5–8). In lanes: 1, 3 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n364 plasmid; 2, 4 - the soluble fraction recovered after centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1n364 plasmid; 5, 7 - whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s364 plasmid; 6, 8 - the soluble fraction recovered after the centrifugation of whole lysate of both yeast clones transformed with pFX7-HPyV12-VP1s364; 9 - purified HPyV12-VP1364 protein; M - pre-stained protein weight marker (Thermo Fisher Scientific Baltics)