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Fig 1 | BMC Biotechnology

Fig 1

From: Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips

Fig 1

IL-20 quantification using plasmonic gold substrates. a) IL-20 standard curves obtained using rh-IL-20 expressed in E.Coli (produced by Novo Nordisk A/S (), or R&D Systems ()) or in HEK-293 (Novo Nordisk A/S ()). rh-IL-20 was diluted in 5 % BSA in PBS. Median ± SD fluorescence intensities are shown. Lower limit of quantification (LLOQ) is indicated by the vertical dotted line. b) Fluorescence intensities for IL-20 and isotype control capture antibodies before and after pre-incubation with a mix of animal IgGs. Serum (black) and synovial fluid (white) mean IL-20 levels ± SD for two donors are shown. Representative maps (inserts) shown for one donor

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