Table 2 Expression vectors used in this study
From: Cold shock induction of recombinant Arctic environmental genes
Vector | Fusion partners | Fusion partner size (kDa)a | Promoter | Linker (aa)b | Resistance gene | Manufacturer |
|---|---|---|---|---|---|---|
pCold-II | His | n.d. | cspA | - | bla | Takara |
pCold-II-TEV | His | 3 | cspA | 16 | bla | This study |
pCold-II-TRX-TEV | His, TRX | 15 | cspA | 11 | bla | This study |
pCold-II-MBP-TEV | His, MBP | 43 | cspA | 11 | bla | This study |
pCold-II-SUMO | His, SUMO | 13 | cspA | 0 | bla | This study |
pCold-II-TF-TEV | His, TF | 51 | cspA | 12 | bla | This study |