Fig. 3
From: Cold shock induction of recombinant Arctic environmental genes
Heterologous expression of candidate genes driven by the cspA promoter. a cspA promoter driven heterologous expression of candidate proteins in three E. coli expression strains; BL21CodonPlus(DE3)RIL (light grey bars), ArcticExpress(DE3)RIL (medium grey bars) and Rosetta2(DE3)pLysS (black bars). A qualitative scale for scoring expression success was used as described in the main text. b Normalized protein expression (upper panel) and solubility (lower panel) of candidates after cspA driven expression in BL21CodonPlus(DE3)RIL. Error bars show the standard deviation between two independent experiments