High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Table 2 Purification of ARG from 200 ml of fermentation supernatant

Step	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Yield (%)
Culture supernatant	302	2458.3	8.14	100
	78.9	8639.6	109.5	351.4
Ni²⁺-affinity chromatography	32.5	8073	248.4	328.4

ISSN: 1472-6750