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Table 2 Purification of ARG from 200 ml of fermentation supernatant

From: High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Step Total protein (mg) Total activity (U) Specific activity (U/mg) Yield (%)
Culture supernatant 302 2458.3 8.14 100
  78.9 8639.6 109.5 351.4
Ni2+-affinity chromatography 32.5 8073 248.4 328.4