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Table 1 Primers used in this study for PCR

From: High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Primers Sequence (5′ → 3′)

 

arg-1 GTCAATGAGTGCTAAGTCCAGAACGATTG

 

arg-2 ATGAGTGCTAAGTCCAGAACGATTG

 

arg-3 CTTAGAAAATGGAGCACCAATAATACCAATCGTTCTGGACTTAGCACTC

 

arg-4 GTATTATTGGTGCTCCATTTTCTAAGGGACAACCAAGAGGTGGTGTCGA

 

arg-5 CCAGCCTTTCTCAAAACTGTTGGACCTTCTTCGACACCACCTCTTGGTT

 

arg-6 AACAGTTTTGAGAAAGGCTGGTCTACTTGAAAAGTTGAAAGAACAAGAA

 

arg-7 ATCACCGTAATCCTTAACATCACATTCTTGTTCTTTCAACTTTTCAAGT

 

arg-8 GTGATGTTAAGGATTACGGTGATTTGCCATTTGCTGATATCCCAAACGA

 

arg-9 GGATTCTTCACAATTTGGAATGGAGAATCGTTTGGGATATCAGCAAATG

 

arg-10 CCATTCCAAATTGTGAAGAATCCAAGATCTGTGGGAAAAGCCTCTGAAC

 

arg-11 CTTAACTTCAGCAACTTTACCAGCCAGCTGTTCAGAGGCTTTTCCCACA

 

arg-12 GCTGGTAAAGTTGCTGAAGTTAAGAAGAACGGTAGAATTTCTCTTGTTT

 

arg-13 TAGCCAAAGAATGATCACCACCCAAAACAAGAGAAATTCTACCGTTCTT

 

arg-14 GGTGGTGATCATTCTTTGGCTATTGGTTCTATTTCAGGACATGCTAGAG

 

arg-15 CAAATAACACCCAAGTCTGGATGAACTCTAGCATGTCCTGAAATAGAAC

 

arg-16 CATCCAGACTTGGGTGTTATTTGGGTTGATGCTCATACTGACATTAACA

 

arg-17 TACCAGAAGTAGTAGTCAGTGGAGTGTTAATGTCAGTATGAGCATCAAC

 

arg-18 TCCACTGACTACTACTTCTGGTAACTTGCATGGTCAACCAGTTTCTTTT

 

arg-19 AATCTTACCCTTCAATTCCTTCAACAAAAAAGAAACTGGTTGACCATGC

 

arg-20 GTTGAAGGAATTGAAGGGTAAGATTCCAGATGTTCCAGGTTTTTCTTGG

 

arg-21 ATCCTTAGCAGATATACATGGAGTAACCCAAGAAAAACCTGGAACATCT

 

arg-22 TACTCCATGTATATCTGCTAAGGATATTGTTTACATCGGTTTGAGAGAT

 

arg-23 AGATGTAATGTTCACCTGGATCAACATCTCTCAAACCGATGTAAACAAT

 

arg-24 TGATCCAGGTGAACATTACATCTTGAAGACTTTGGGTATTAAGTACTTT

 

arg-25 AACCTATCAACTTCGGTCATAGAAAAGTACTTAATACCCAAAGTCTTCA

 

arg-26 TCTATGACCGAAGTTGATAGGTTGGGAATTGGCAAGGTTATGGAAGAAA

 

arg-27 CTTTCTACCCAACAAGTAAGACAATGTTTCTTCCATAACCTTGCCAATT

 

arg-28 ATTGTCTTACTTGTTGGGTAGAAAGAAGAGACCAATCCATTTGTCTTTT

 

arg-29 AAGATGGGTCCAAACCATCAACGTCAAAAGACAAATGGATTGGTCTCTT

 

arg-30 TTGATGGTTTGGACCCATCTTTCACTCCAGCTACTGGTACTCCAGTTGT

 

arg-31 CAAACCCTCTCTGTACGTTAGACCACCAACAACTGGAGTACCAGTAGCT

 

arg-32 TCTAACGTACAGAGAGGGTTTGTACATTACTGAGGAGATTTACAAAACA

 

arg-33 ATATCCAAGCCAGACAACAAACCTGTTTTGTAAATCTCCTCAGTAATGT

 

arg-34 GTTTGTTGTCTGGCTTGGATATTATGGAGGTTAATCCATCCTTGGGCAA

 

arg-35 TACCGTACGAGTAACTTCTTCTGGAGTCTTGCCCAAGGATGGATTAACC

 

arg-36 CAGAAGAAGTTACTCGTACGGTAAACACTGCCGTTGCAATTACATTAGC

 

arg-37 GTTACCTTCACGAGCTAAACCGAAGCAAGCTAATGTAATTGCAACGGCA

 

arg-38 CGGTTTAGCTCGTGAAGGTAACCATAAACCAATTGACTATTTGAACCCA

 

arg-39 CACAATTTTAATGATGATGATGATGGTGCTTTGGTGGGTTCAAATAGTCAATTGGTTTA

 

arg-40 GGCCATTAATGATGATGATGATGGGTGCTTTGGTGGGTTCAAATAGTCAATTGGTTTA

 
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BMC Biotechnology

ISSN: 1472-6750

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