Fig. 3
From: Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Expression of His-tag insertion and terminal fusion CCMV constructs in E. coli. a Cell extracts (CE) and reassembled VLP pellets (RP) collected through sucrose cushion of CCMV-CP (A 1) and CCMP-CP derivatives containing a 6xHis-tag insertion within each of the loop structures (βB-βC-His [A 2], βD-βE-His[A 3], βF-βG-His[A 4] and βH-βI-His[A 5]), were resolved on 15 % SDS-PAGE and stained with Coomassie blue. b Western blots of 6xHis-tag fusion constructs (His-CP, CP-His, His-CP-His) detected by polyclonal anti-CCMV (B 1) and monoclonal anti-His (B 2) sera. CCMV-CP and NΔ24-CP were used as controls for size comparison with the chimeras. A molecular size marker is indicated on the left