Table 1 List of primers used in PCR
From: Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci
Name | Sequence | Used for |
|---|---|---|
TLR7F | 5′-CAC GCC GAA AGT TTC ATC TA-3′ | cDNA PCR |
TLR7R | 5′-TTG TCG TTC AAA AGG AGGG-3′ | cDNA PCR |
TLR7F-1 | 5′-CCG CTCGAG CAC GCC GAA AGT TTC ATC TA-3′ XhoI | Forward fragment PCR |
TLR7R-1 | 5′-AGC AAGCTT TTG TCG TTC AAA AGG AGGG-3′ HindIII | Forward fragment PCR |
TLR7F-2 | 5′-CGG GGTACC CAC GCC GAA AGT TTC ATC TA-3′ KpnI | Reverse fragment PCR |
TLR7R-2 | 5′-CAT GCATGC TTG TCG TTC AAA AGG AGGG-3′ SphI | Reverse fragment PCR |
HygB-F | 5′-CGA CAG CGT CTC CGA CCTGA-3′ | PCR of hygromycin B phosphotransferase gene |
HygB-R | 5′-TTC GAT GAT GCA GCT TGG GCG-3′ | PCR of hygromycin B phosphotransferase gene |
TLR7-VF | 5′-GTCACCGACGAAATCC-3′ | Validation for expression of TLR7 gene of whitefly |
TLR7-VR | 5′-AGAGTCCCAGCCTTGTT-3′ | Validation for expression of TLR7 gene of whitefly |
β-actin-F | 5′-TCACCACCACAGCTGAGAGA-3′ | Validation for expression of TLR7 gene of whitefly |
β-actin-R | 5′-CTCGTGGATACCGCAAGATT-3′ | Validation for expression of TLR7 gene of whitefly |