Skip to main content

Table 1 List of primers used in PCR

From: Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci

Name Sequence Used for
TLR7F 5′-CAC GCC GAA AGT TTC ATC TA-3′ cDNA PCR
TLR7R 5′-TTG TCG TTC AAA AGG AGGG-3′ cDNA PCR
TLR7F-1 5′-CCG CTCGAG CAC GCC GAA AGT TTC ATC TA-3′ XhoI Forward fragment PCR
TLR7R-1 5′-AGC AAGCTT TTG TCG TTC AAA AGG AGGG-3′ HindIII Forward fragment PCR
TLR7F-2 5′-CGG GGTACC CAC GCC GAA AGT TTC ATC TA-3′ KpnI Reverse fragment PCR
TLR7R-2 5′-CAT GCATGC TTG TCG TTC AAA AGG AGGG-3′ SphI Reverse fragment PCR
HygB-F 5′-CGA CAG CGT CTC CGA CCTGA-3′ PCR of hygromycin B phosphotransferase gene
HygB-R 5′-TTC GAT GAT GCA GCT TGG GCG-3′ PCR of hygromycin B phosphotransferase gene
TLR7-VF 5′-GTCACCGACGAAATCC-3′ Validation for expression of TLR7 gene of whitefly
TLR7-VR 5′-AGAGTCCCAGCCTTGTT-3′ Validation for expression of TLR7 gene of whitefly
β-actin-F 5′-TCACCACCACAGCTGAGAGA-3′ Validation for expression of TLR7 gene of whitefly
β-actin-R 5′-CTCGTGGATACCGCAAGATT-3′ Validation for expression of TLR7 gene of whitefly