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Fig 5 | BMC Biotechnology

Fig 5

From: Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva

Fig 5

Western Blot analysis of recombinantly expressed tick salivary proteins metalloprotease 1 a, Ubiquitin Ligase b and Thrombin Inhibitor c. All three proteins were expressed as His-Tag fusion proteins and detected with an anti-His-Tag antibody. Abbreviations represent the following: CL crude lysate, SN supernatant after binding to Ni-Sepharose, 1 W first wash fraction, 2 W second wash fraction, 3 W third wash fraction, PBSW PBS wash fraction, 1E first eluted fraction, 2E second eluted fraction, 3E third eluted fraction

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BMC Biotechnology

ISSN: 1472-6750

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