Table 1 Primers used for the manipulation of ku70 and the identification of its mutants
From: Development of genetic tools for Myceliophthora thermophila
Names | Primer sequences (5′-3′) | Notes |
|---|---|---|
bar-F | CTGGAGCTAGTGGAGGTCAAC | PCR detection of bar |
bar-R | TTCAATCTTAAGAAACTTTATTG | PCR detection of bar |
ku70-5 F | GCTCTAGAGCGCTACCACGGACGGATGATAC | Cloning ku70 5’flank |
ku70-5R | CCGGAATTCGGCTTTGAAGGTGCAGGTGCGAC | Cloning ku70 5’flank |
ku70-3 F | GGACTAGTGTTGGGATCGCATGGTTCGTTG | Cloning ku70 3’flank |
ku70-3R | CCGATATCGGCTTCAGAATGCAGAGGTCAGAG | Cloning ku70 3’flank |
Probe-F | GCGACGATCCCGAAATAC | Southern blotting of ku70 |
Probe-R | CCCTGACGAAGTAGCATCAT | Southern blotting of ku70 |
ku70KO-F | CTACACCCACCTGCTGAAGTCC | PCR detection of ku70 mutation |
ku70KO-R | GTCCCGCACTACCGTTGATGG | PCR detection of ku70 mutation |
ku70-F | CGTGGGAAGGTGACGATGATAGAC | PCR detection of ku70 ORF |
ku70-R | CATAATGCTCCTCGCTCGGGAAG | PCR detection of ku70 ORF |
PtrpC-F | AAAAAGCTTGGTACCGAGCTCCGACGTTAACTGATATTGAAGGAGC | Cloning PtrpC |
PtrpC-R | CGTGCAATCCATCTTGTTCAATCATTTGGATGCTTGGGTAGAATAGGTAA | Cloning PtrpC |
neo-F | TTACCTATTCTACCCAAGCATCCAAATGATTGAACAAGATGGATTGCACG | Cloning neo |
neo-R | AAATTAATTAAGTTTAAACCTCGAGTCTAGAAGATCTTCAGAAGAACTCGTCAAGAAGGCGA | Cloning neo |
pyrG-5 F | AAAGAATTCCGATGCAGATGCAACTCCGCTCCCT | Cloning pyrG 5’flank |
pyrG-5R | AAAGGATCCAGGTTCGAGGCCTTGAGGTCCATGA | Cloning pyrG 5’flank |
pyrG-3 F | CCGCTCGAGATCTGGCGCGTCTGGCGTGATTTGG | Cloning pyrG 3’flank |
pyrG-3R | GGAAGATCTCGGGGTGAGTGTTGGGGTGTTGTTT | Cloning pyrG 3’flank |
pyrGout-F | GGGCTGACCGCTTCCTCGTGCTTTA | PCR detection of pyrG |
pyrGout-R | TGGCGTAGTGCGTGTTGAACTCGGC | PCR detection of pyrG |